Mapping Polarization Fields in Al0.85In0.15N/AlN/GaN Heterostructures

Extended abstract of a paper presented at Microscopy and Microanalysis 2009 in Richmond, Virginia, USA, July 26 - July 30, 200

A child presenting with acute renal failure secondary to a high dose of indomethacin: a case report

<p>Abstract</p> <p>Introduction</p> <p>Acute renal failure caused by nonsteroidal anti-inflammatory drugs administered at therapeutic doses is generally mild, non-anuric and transitory. There are no publications on indomethacin toxicity secondary to high doses in children. The aim of this article is to describe acute renal failure secondary to a high dose of indomethacin in a child and to review an error in a supervised drug prescription and administration system.</p> <p>Case presentation</p> <p>Due to a medication error, a 20-day-old infant in the postoperative period of surgery for Fallot's tetralogy received a dose of 10 mg/kg of indomethacin, 50 to 100 times higher than the therapeutic dose. The child presented with acute, oligo-anuric renal failure requiring treatment with continuous venovenous renal replacement therapy, achieving complete recovery of renal function with no sequelae.</p> <p>Conclusion</p> <p>In order to reduce medication errors in critically ill children, it is necessary to develop a supervised drug prescription and administration system, with controls at various levels.</p

Highly efficient catalysis of the Kemp elimination in the cavity of a cubic coordination cage.

The hollow cavities of coordination cages can provide an environment for enzyme-like catalytic reactions of small-molecule guests. Here, we report a new example (catalysis of the Kemp elimination reaction of benzisoxazole with hydroxide to form 2-cyanophenolate) in the cavity of a water-soluble M8L12 coordination cage, with two features of particular interest. First, the rate enhancement is among the largest observed to date: at pD 8.5, the value of kcat/kuncat is 2 × 10(5), due to the accumulation of a high concentration of partially desolvated hydroxide ions around the bound guest arising from ion-pairing with the 16+ cage. Second, the catalysis is based on two orthogonal interactions: (1) hydrophobic binding of benzisoxazole in the cavity and (2) polar binding of hydroxide ions to sites on the cage surface, both of which were established by competition experiments

The VANDELS Survey: New constraints on the high-mass X-ray binary populations in normal star-forming galaxies at 3 < z < 5.5

We use VANDELS spectroscopic data overlapping with the ≃7 Ms Chandra Deep Field South survey to extend studies of high-mass X-ray binary systems (HMXBs) in 301 normal star-forming galaxies in the redshift range 3 6 may be ≳0.25 dex higher than previously estimated

A Recoding Method to Improve the Humoral Immune Response to an HIV DNA Vaccine

This manuscript describes a novel strategy to improve HIV DNA vaccine design. Employing a new information theory based bioinformatic algorithm, we identify a set of nucleotide motifs which are common in the coding region of HIV, but are under-represented in genes that are highly expressed in the human genome. We hypothesize that these motifs contribute to the poor protein expression of gag, pol, and env genes from the c-DNAs of HIV clinical isolates. Using this approach and beginning with a codon optimized consensus gag gene, we recode the nucleotide sequence so as to remove these motifs without modifying the amino acid sequence. Transfecting the recoded DNA sequence into a human kidney cell line results in doubling the gag protein expression level compared to the codon optimized version. We then turn both sequences into DNA vaccines and compare induced antibody response in a murine model. Our sequence, which has the motifs removed, induces a five-fold increase in gag antibody response compared to the codon optimized vaccine

A human MAP kinase interactome.

Mitogen-activated protein kinase (MAPK) pathways form the backbone of signal transduction in the mammalian cell. Here we applied a systematic experimental and computational approach to map 2,269 interactions between human MAPK-related proteins and other cellular machinery and to assemble these data into functional modules. Multiple lines of evidence including conservation with yeast supported a core network of 641 interactions. Using small interfering RNA knockdowns, we observed that approximately one-third of MAPK-interacting proteins modulated MAPK-mediated signaling. We uncovered the Na-H exchanger NHE1 as a potential MAPK scaffold, found links between HSP90 chaperones and MAPK pathways and identified MUC12 as the human analog to the yeast signaling mucin Msb2. This study makes available a large resource of MAPK interactions and clone libraries, and it illustrates a methodology for probing signaling networks based on functional refinement of experimentally derived protein-interaction maps

The VANDELS survey: Global properties of CIII]lambda 1908 angstrom emitting star-forming galaxies at z similar to 3

Context. Strong nebular emission is ubiquitous in galaxies that contribute to cosmic reionization at redshift za a 6. High-ionization UV metal lines, such as CIII]λ1908 A, show high equivalent widths (EW) in these early galaxies, suggesting harder radiation fields at low metallicity than low-z galaxies of similar stellar mass. Understanding the physical properties driving the observed UV nebular line emission at high-z requires large and very deep spectroscopic surveys, which are now only accessible out to za a a 4. Aims. We study the mean properties of a large representative sample of 217 galaxies showing CIII] emission at 2a <a za <a 4, selected from a parent sample of a 750 main-sequence star-forming galaxies in the VANDELS survey. These CIII] emitters have a broad range of UV luminosities, allowing for a detailed stacking analysis to characterize their stellar mass, star formation rate (SFR), and metallicity as a function of the UV emission line ratios, EWs, and the carbon-to-oxygen (C/O) abundance ratio. Methods. Stacking provides unprecedented high signal-to-noise (S/N) spectra for CIII] emitters over more than three decades in luminosity, stellar mass, and SFR. This enables a full spectral fitting to derive stellar metallicities for each stack. Moreover, we use diagnostics based on photoionization models and UV line ratios to constrain the ionization sources of the galaxies and derive the C/O abundance. Results. Reliable CIII] detections (S/Na a ¥a 3) represent a 30% of the parent sample. However, stacked spectra of non-detections (S/Na <a 3) show weak (EW a 2 A) CIII] emission, suggesting that this line is common in normal star-forming galaxies at za a a 3. On the other hand, extreme CIII] emitters (EW(CIII]) a 8 A) are exceedingly rare (a 3%) in VANDELS. The UV line ratios of the sample suggest no ionization source other than massive stars. Stacks with larger EW(CIII]) show larger EW(Lyα) and lower metallicity, but not all CIII] emitters are Lyα emitters. The stellar metallicities of CIII] emitters are not significantly different from that of the parent sample, increasing from a 10% to a 40% solar for stellar masses log(Ma/Ma) a 9a? 10.5. The stellar mass-metallicity relation of the CIII] emitters is consistent with previous works, exhibiting a strong evolution from za =a 0 to za a a 3. The C/O abundances of the sample range between 35%a? 150% solar, with a noticeable increase with FUV luminosity and a smooth decrease with the CIII] EW. Here, we discuss the CIII] emitters in the C/Oa Fe/H and the C/Oa O/H planes and we find that they follow stellar and nebular abundance trends consistent with those of Milky Way halo and thick-disk stars and local HII galaxies, respectively. A qualitative agreement is also found with chemical evolution models, which suggests that CIII] emitters at za a a 3 are experiencing an active phase of chemical enrichment. Conclusions. Our results provide new insights into the nature of UV line emitters at za a a 2a 4, paving the way for future studies at higher z using the James Webb Space Telescope

Gender differences in the association between adiposity and probable major depression: a cross-sectional study of 140,564 UK Biobank participants

&lt;b&gt;Background&lt;/b&gt;&lt;p&gt;&lt;/p&gt; Previous studies on the association between adiposity and mood disorder have produced contradictory results, and few have used measurements other than body mass index (BMI). We examined the association between probable major depression and several measurements of adiposity: BMI, waist circumference (WC), waist-hip-ratio (WHR), and body fat percentage (BF%).&lt;p&gt;&lt;/p&gt; &lt;b&gt;Methods&lt;/b&gt;&lt;p&gt;&lt;/p&gt; We conducted a cross-sectional study using baseline data on the sub-group of UK Biobank participants who were assessed for mood disorder. Multivariate logistic regression models were used, adjusting for potential confounders including: demographic and life-style factors, comorbidity and psychotropic medication.&lt;p&gt;&lt;/p&gt; &lt;b&gt;Results&lt;/b&gt;&lt;p&gt;&lt;/p&gt; Of the 140,564 eligible participants, evidence of probable major depression was reported by 30,145 (21.5%). The fully adjusted odds ratios (OR) for obese participants were 1.16 (95% confidence interval (CI) 1.12, 1.20) using BMI, 1.15 (95% CI 1.11, 1.19) using WC, 1.09 (95% CI 1.05, 1.13) using WHR and 1.18 (95% CI 1.12, 1.25) using BF% (all p &#60;0.001). There was a significant interaction between adiposity and gender (p = 0.001). Overweight women were at increased risk of depression with a dose response relationship across the overweight (25.0-29.9 kg/m2), obese I (30.0-34.9 kg/m2), II (35.0-39.9 kg/m2) and III (≥40.0 kg/m2) categories; fully adjusted ORs 1.14, 1.20, 1.29 and 1.48, respectively (all p &#60; 0.001). In contrast, only obese III men had significantly increased risk of depression (OR 1.29, 95% CI 1.08, 1.54, p = 0.006).&lt;p&gt;&lt;/p&gt; &lt;b&gt;Conclusion&lt;/b&gt;&lt;p&gt;&lt;/p&gt; Adiposity was associated with probable major depression, irrespective of the measurement used. The association was stronger in women than men. Physicians managing overweight and obese women should be alert to this increased risk

Pharmacogenetic prediction of clinical outcome in advanced colorectal cancer patients receiving oxaliplatin/5-fluorouracil as first-line chemotherapy

To determine whether molecular parameters could be partly responsible for resistance or sensitivity to oxaliplatin (OX)-based chemotherapy used as first-line treatment in advanced colorectal cancer (CRC). We studied the usefulness of the excision repair cross-complementing 1 (ERCC1), xeroderma pigmentosum group D (XPD), XRCC1 and GSTP1 polymorphisms as predictors of clinical outcome in these patients. We treated 126 CRC patients with a first-line OX/5-fluorouracil chemotherapeutic regimen. Genetic polymorphisms were determined by real-time PCR on an ABI PRISM 7000, using DNA from peripheral blood. Clinical response (CR), progression-free survival (PFS) and overall survival (OS) were evaluated according to each genotype. In the univariate analysis for CR, ERCC1-118 and XPD 751 polymorphisms were significant (P=0.02 and P=0.05, respectively). After adjustment for the most relevant clinical variables, only ERCC1-118 retained significance (P=0.008). In the univariate analysis for PFS, ERCC1-118 and XPD 751 were significant (P=0.003 and P=0.009, respectively). In the multivariant analysis, only the XPD 751 was significant for PFS (P=0.02). Finally, ERCC1-118 and XPD 751 polymorphisms were significant in the univariate analysis for OS (P=0.006 and P=0.015, respectively). Both genetic variables remained significant in the multivariate Cox survival analysis (P=0.022 and P=0.03). Our data support the hypothesis that enhanced DNA repair diminishes the benefit of platinum-based treatments